PreScissionProtease是一种由人鼻病毒14型的3C蛋白酶(humanrhinovirus(HRV)type143Cprotease)和GST组成的融合蛋白。该蛋白酶可在低温下(4°C)特异识别短肽Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro,并在Gln和Gly氨基酸残基之间进行酶切。底物的识别和切割不仅依赖于融合蛋白的一级结构还依赖于融合蛋白的二级和三级结构。它可以特异性的将pGEX-6P系列等载体表达出的带有酶底物识别多肽序列融合蛋白的GST标签进行分离。本品由大肠杆菌中重组表达,以无菌液体形式提供。产品性质中文别名(ChineseSynonym)重组Prescission蛋白酶英文别名(EnglishSynonym)3Cprotease,Picornain3C,PSP来源(Source)大肠杆菌表达分子量(MolecularWeight)约46kDa物理外观(PhysicalAppearance)无菌无色液体储存缓冲液(StorageBuffer)25mMTris-HCl(pH8.0),150mMNaCl,1mMEDTA,5mMDTT,50%(V/V)Glycerin2aR蛋白在细胞内发挥着不同的作用,例如在信号传递、物质转运和细胞通讯等方面。Recombinant Mouse EPHA10 Protein,His Tag

α-凝血酶(α-Thrombin)是一种高度特异性的丝氨酸蛋白酶,由凝血酶原(Prothrombin,II因子)经蛋白水解活化而来。它在凝血过程中起着非常重要的作用,不仅能够促使纤维蛋白凝块的产生,还负责提供反馈信号来寻找前辅因子:V因子和VIII因子。也可以用作血管收缩剂。在体内,活化的X因子(FactorXa)切割凝血酶原,释放出活性肽并将凝血酶切割成具有催化活性的α-凝血酶。α-凝血酶由一条轻链(Achain)(Mw~6,000)和一条重链(Bchain)(Mw~31,000)通过二硫键连接而成。某些情况下,α-凝血酶会发生自溶生成β-凝血酶和γ-凝血酶。β-凝血酶由对α-凝血酶A链水解并切割含有B链糖基化位点的小片段(B1,B2)组合而成。除了用于凝血研究之外,α-凝血酶还常用来位点特异性切割融合蛋白。基因重组时将凝血酶识别位点插入在目的蛋白与利于后续纯化和/或表达的多肽或者蛋白之间,通过凝血酶切割表达的重组子即可释放目的蛋白。凝血酶本身可通过亲和层析技术快速去除。本品是由匀质化的人凝血酶原经由Xa因子,Va因子和磷脂活化而得,经SDS-PAGE检测确保凝血酶原的完全活化,并以NIH凝血酶的标准品为参考,提供的酶活力不少于3091NIHU/mg。Recombinant Canine AFP Protein,His Tag透明质酸(Hyaluronic Acid,简称HA)是一种存在于人体和动物组织中的天然高分子多糖,称为糖醛酸或玻尿酸。

RecombinantHumanACE2/ACEHProtein,hFcTag性能参数,分子别名(Synonyms)ACE2;ACEH;ACE-2表达区间及表达系统(Source)HumanACE2/ACEHProteinisexpressedfromHEK293withhFctagattheC-Terminus.ItcontainsGln18-Ser740.[Accession|Q9BYF1-1]分子量大小(MolecularWeight)TheproteinhasapredictedMWof109.2kDa.Duetoglycosylation,theproteinmigratesto115-130kDabasedonSDS-PAGEresult.Endotoxin)Lessthan1EUperμgbytheLALmethod.纯度(Purity)>95%asdeterminedbySDS-PAGEandHPLC.活性(Activity)ELISAData:ImmobilizedSARS-COV-2SpikeS(B.1.1.529/Omicron)Trimer,HisTagat1μg/ml(100μl/well)ontheplate.DoseresponsecurveforHumanACE2,hFcTagwiththeEC50of13.7ng/mldeterminedbyELISA.制剂(Formulation)Lyophilizedfrom0.22μmfilteredsolutioninPBS(pH7.4).Normally8%trehaloseisaddedasprotectantbeforelyophilization.重构方法(Reconstitution)Centrifugethetubebeforeopening.Reconstitutingtoaconcentrationmorethan100μg/mlisrecommended.Dissolvethelyophilizedproteinindistilledwater.
性能参数表达区间及表达系统(Source)CynomolgusCD27Ligand/CD70ProteinisexpressedfromHEK293withHistagattheN-terminal.ItcontainsGln39-Pro194.[Accession|G7PYU6-1]分子量大小(MolecularWeight)TheproteinhasapredictedMWof18.4kDa.Duetoglycosylation,theproteinmigratesto60-90kDabasedonTris-BisPAGEresult.(Endotoxin)Lessthan1EUperugbytheLALmethod.纯度(Purity)>95%asdeterminedbyTris-BisPAGE活性(Activity)ELISAData:ImmobilizedCynomolgusCD27Ligand,HisTagat2μg/ml(100μl/well)ontheplate.DoseresponsecurveforCynomolgus/RhesusmacaqueCD27,hFcTagwiththeEC50of70.8ng/mldeterminedbyELISA.制剂(Formulation)Lyophilizedfrom0.22μmfilteredsolutioninPBS(pH7.4).Normally8%trehaloseisaddedasprotectantbeforelyophilization.重构方法(Reconstitution)Centrifugetubesbeforeopening.Reconstitutingtoaconcentrationmorethan100μg/mlisrecommended.Dissolvethelyophilizedproteinindistilledwater.由于其在细胞外基质中的存在,透明质酸在细胞黏附、迁移、增殖和分化过程中扮演着关键角色。

RecombinantBiotinylatedHumanAFP(HLA-A*02:03)Protein,His-AviTag性能参数表达区间及表达系统(Source)RecombinantBiotinylatedHumanAFP(HLA-A*02:03)ProteinisexpressedfromHEK293withHistagandAvitagattheC-terminal..ItcontainsGly25-Thr305(HLA-A*02:03),Ile21-Met119(B2M)andFMNKFIYEIpeptide.[Accession|AAA03604.1(HLA-A*02:03)&P61769(B2M)&FMNKFIYEI]分子量大小(MolecularWeight)TheproteinhasapredictedMWof50.70kDa.Duetoglycosylation,theproteinmigratesto53-60kDabasedonTris-BisPAGEresult.(Endotoxin)Lessthan1EUperμgbytheLALmethod.纯度(Purity)>95%asdeterminedby>95%asdeterminedbyHPLC制剂(Formulation)Lyophilizedfrom0.22μmfilteredsolutioninPBS(pH7.4).Normally8%trehaloseisaddedasprotectantbeforelyophilization.重构方法(Reconstitution)Centrifugetubesbeforeopening.Reconstitutingtoaconcentrationmorethan100μg/mlisrecommended.Dissolvethelyophilizedproteinindistilledwater.在生物制药工业中,肠激酶用于生产重组蛋白质药物,通过专一性切割去除不需要的序列,提高药物纯度和活性。Recombinant Mouse IL-33
由于其在细胞信号传递中的重要性,A2aR成为了药物开发的重要靶点之一。Recombinant Mouse EPHA10 Protein,His Tag
SARS-CoV-2,whichcausestheglobalpandemiccoronavirusdisease2019(Covid-19),belongstoafamilyofvirusesknownascoronavirusesthatalsoincludeMERS‑CoVandSARS-CoV-1.Coronavirusesarecommonlycomprisedoffourstructuralproteins:Spikeprotein(S),Envelopeprotein(E),Membraneprotein(M)andNucleocapsidprotein(N).TheSARS-CoV-2Sproteinisaglycoproteinthatmediatesmembranefusionandviralentry.TheSproteinishomotrimeric,witheach~180-kDamonomerconsistingoftwosubunits,S1andS2.TheRBDofSARS-CoV-2bindsametallopeptidase,angiotensin-convertingenzyme2(ACE-2).BeforebindingtotheACE-2receptor,structuralanalysisoftheS1trimershowsthatonlyoneofthethreeRBDdomainsisinthe"up"conformation.Thisisanunstableandtransientstatethatpassesbetweentrimericsubunitsbutisneverthelessanexposedstatetobetargetedforneutralizingantibodytherapy.PolyclonalantibodiestotheRBDoftheSARS-CoV-2proteinhavebeenshowntoinhibitinteractionwiththeACE-2receptor,confirmingRBDasanattractivetargetforvaccinationsorantiviraltherapy.Recombinant Mouse EPHA10 Protein,His Tag
DL2000DNAMarker:分子量标准的可靠选择DL2000DNAMarker是一种广应用于琼脂糖凝胶电泳的DNA分子量标准,由6条线状双链DNA片段组成,适用于常规DNA片段大小的鉴定。产品特点组成:DL2000DNAMarker包含100bp、250bp、500bp、750bp、1000bp、2000bp共6条DNA片段。清晰度高:条带清晰锐利,背景干净,亮度均匀。稳定性好:在室温下可稳定存放三个月以上,长期保存建议置于-20℃。即用型设计:已预混1×LoadingBuffer,可直接上样。用场景DL2000DNAMarker适用于常规PCR产物、基因组DNA片段等的大小鉴定,是分子生...