PE-labeledHumanHLA-A*02:01&B2M&P53R175H(HMTEVVRHC)TetramerProtein,His-AviTag性能参数表达区间及表达系统(Source)PE-labeledHumanHLA-A*02:01&B2M&P53R175H(HMTEVVRHC)TetramerProteinisexpressedfromHEK293withHistagandAvitagattheC-Terminus.PE-labeledHumanHLA-A*02:01&B2M&P53R175H(HMTEVVRHC)TetramerisassembledbybiotinylatedmonomerandPE-labeledstreptavidin.ItcontainsGly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)andHMTEVVRHCpeptide.(Endotoxin)Lessthan1EUperμgbytheLALmethod.制剂(Formulation)Suppliedas0.22μmfilteredsolutionin20mMPB,500mMNaCl,0.2%BSA(pH8.0).储存条件Theproductshouldbestoredat-85~-65℃for3monthfromdateofreceipt.Recommendtoaliquottheproteinintosmallerquantitieswhenfirstusedandavoidrepeatedfreeze-thawcycles.透明质酸的生产可以通过动物组织提取、微生物发酵或化学合成等方法进行。Recombinant Human IL-7R alpha/CD127 Protein,hFc Tag
Coronavirusesarecommonlycomprisedoffourstructuralproteins:Spikeprotein(S),Envelopeprotein(E),Membraneprotein(M)andNucleocapsidprotein(N).TheSARS-CoV-2Sproteinisaglycoproteinthatmediatesmembranefusionandviralentry.TheSproteinishomotrimeric,witheach~180-kDamonomerconsistingoftwosubunits,S1andS2.TheRBDofSARS-CoV-2bindsametallopeptidase,angiotensin-convertingenzyme2(ACE-2).产品性质别名SproteinRBD;SglycoproteinRBD;SpikeproteinRBDAccessionQHD43416.1表达区间及表达系统RecombinantSARS-CoV-2SpikeRBD(OmicronBA.4/BA.5/BA.5.1.3/BA.5.2)ProteinisexpressedfromExpi293withHistagattheC-terminal.ItcontainsArg319-Phe541(G339D,S371F,S373P,S375F,T376A,D405N,R408S,K417N,N440K,L452R,S477N,T478K,E484A,F486V,Q498R,N501Y,Y505H).Recombinant Human IFN gamma R1/IFNGR1 Protein,hFc TagPNGase F是一种酰胺水解酶,能够特异性地裂解糖蛋白中由天冬酰胺连接的高甘露糖、杂合和复杂型的寡糖。
利用PCR技术扩增得到编码土豆羧肽酶抑制剂(carboxypeptidaseinhibitorfrompotato,CPI)活性多肽基因,克隆于表达载体pGEX一4T一1的多克隆位点中,得到重组质粒pGCPI,测序后将重组质粒转化到受体茵EscherichiacoliBL21中。重组茵经IPTG诱导表达,超声波破茵后,通过谷胱甘肽sepheroseFF亲和层析柱一步纯化得到融合蛋白,纯度大于97%。融合蛋白经凝血酶切割并分离除去谷胱甘肽一S一转移酶后得到纯度大于98%的重组CPI,ELISA鉴定产物具有免疫原性,体外检测表明其促进纤溶的生物功能与天然CPI无明显差异。
RecombinantHumanACE2/ACEHProtein,hFcTag性能参数,分子别名(Synonyms)ACE2;ACEH;ACE-2表达区间及表达系统(Source)HumanACE2/ACEHProteinisexpressedfromHEK293withhFctagattheC-Terminus.ItcontainsGln18-Ser740.[Accession|Q9BYF1-1]分子量大小(MolecularWeight)TheproteinhasapredictedMWof109.2kDa.Duetoglycosylation,theproteinmigratesto115-130kDabasedonSDS-PAGEresult.Endotoxin)Lessthan1EUperμgbytheLALmethod.纯度(Purity)>95%asdeterminedbySDS-PAGEandHPLC.活性(Activity)ELISAData:ImmobilizedSARS-COV-2SpikeS(B.1.1.529/Omicron)Trimer,HisTagat1μg/ml(100μl/well)ontheplate.DoseresponsecurveforHumanACE2,hFcTagwiththeEC50of13.7ng/mldeterminedbyELISA.制剂(Formulation)Lyophilizedfrom0.22μmfilteredsolutioninPBS(pH7.4).Normally8%trehaloseisaddedasprotectantbeforelyophilization.重构方法(Reconstitution)Centrifugethetubebeforeopening.Reconstitutingtoaconcentrationmorethan100μg/mlisrecommended.Dissolvethelyophilizedproteinindistilledwater.全长A2aR蛋白具有天然完整构象,VLP(病毒样颗粒)固有特征可以带来更高的免疫原性。
PreScissionProtease是一种由人鼻病毒14型的3C蛋白酶(humanrhinovirus(HRV)type143Cprotease)和GST组成的融合蛋白。该蛋白酶可在低温下(4°C)特异识别短肽Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro,并在Gln和Gly氨基酸残基之间进行酶切。底物的识别和切割不仅依赖于融合蛋白的一级结构还依赖于融合蛋白的二级和三级结构。它可以特异性的将pGEX-6P系列等载体表达出的带有酶底物识别多肽序列融合蛋白的GST标签进行分离。本品由大肠杆菌中重组表达,以无菌液体形式提供。产品性质中文别名(ChineseSynonym)重组Prescission蛋白酶英文别名(EnglishSynonym)3Cprotease,Picornain3C,PSP来源(Source)大肠杆菌表达分子量(MolecularWeight)约46kDa物理外观(PhysicalAppearance)无菌无色液体储存缓冲液(StorageBuffer)25mMTris-HCl(pH8.0),150mMNaCl,1mMEDTA,5mMDTT,50%(V/V)Glycerin重组人泛素是一种蛋白质,它是一种含有76个氨基酸的高度保守的蛋白质,存在于细胞核和细胞质中。Recombinant Mouse SLAMF1/SLAM/CD150 Protein,His Tag
在某些疾病中,特定蛋白质的泛素化水平可能发生变化,因此,泛素化蛋白质可以作为疾病的生物标志物。Recombinant Human IL-7R alpha/CD127 Protein,hFc Tag
RecombinantBiotinylatedHumanKIR2DL1Protein,His-AviTag性能参数分子别名(Synonyms)CD158A;CD158Ankat1;cl-42表达区间及表达系统(Source)BiotinylatedHumanKIR2DL1ProteinisexpressedfromHEK293withHistagandAvitagattheC-Terminus.ItcontainsHis22-Arg242.[Accession|P43626]分子量大小(MolecularWeight)TheproteinhasapredictedMWof27.1kDa.Duetoglycosylation,theproteinmigratesto48-60kDabasedonSDS-PAGEresult.(Endotoxin)Lessthan1EUperμgbytheLALmethod.纯度(Purity)>95%asdeterminedbySDS-PAGEandHPLC.制剂(Formulation)Lyophilizedfrom0.22μmfilteredsolutioninPBS(pH7.4).Normally8%trehaloseisaddedasprotectantbeforelyophilization.重构方法(Reconstitution)Centrifugethetubebeforeopening.Reconstitutingtoaconcentrationmorethan100μg/mlisrecommended.Dissolvethelyophilizedproteinindistilledwater.储存条件Theproductshouldbestoredat-25~-15℃for1yearfromdateofreceipt.2-7days,2~8°Cundersterileconditionsafterreconstitution.Recombinant Human IL-7R alpha/CD127 Protein,hFc Tag
Lambda核酸外切酶(LambdaExonuclease,简称λExonuclease)是一种具有特定特性和应用的酶,以下是其主要特点和应用:1.**特异性作用**:λExonuclease是一种5'→3'核酸外切酶,能选择性地沿5'→3'方向消化5'端磷酸化的双链DNA。2.**酶切活性**:对单链DNA和5'端未磷酸化修饰的DNA的酶切活性较低,不能从DNA的切刻或缺口处起始消化。3.**嗜磷酸性**:该酶具有非常强的嗜磷酸性,磷酸化的核酸链与非磷酸化的核酸链的切割效率相差达200倍以上。4.**切割效率**:Lambda核酸外切酶是一种具有高度持续性的碱性核酸外切酶,可以连续地将核酸切...